Cell atlas - TRMT12 - The Human Protein Atlas

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TRMT12

TISSUE

CELL

PATHOLOGY

CELL ATLAS RNA EXPRESSION HUMAN CELLS CUSTOM DATA

GENE/PROTEIN

Antibody validation

Dictionary
	Mitochondria
	Nucleoplasm

Human cell
	Mitochondria
	Nucleoplasm

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GENERAL INFORMATION
Gene name	TRMT12
Gene description	TRNA methyltransferase 12 homolog (S. cerevisiae)
Protein class	Enzymes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	1
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm. In addition localized to the mitochondria.
RNA cell category	Cell line enhanced (MCF7)
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (approved)
Additional location	In addition localized to the Mitochondria (approved)
Single-cell variation	Single-cell variation in protein expression observed.
Cell cycle dependency	Cell cycle dependent expression determined by custom assay

DATA RELIABILITY
Reliability score	Approved based on 1 antibody. HPA023939
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Cell line enhanced (MCF7) Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm. In addition localized to the mitochondria.

Main location

Nucleoplasm (approved)

Additional location

Mitochondria (approved)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	HPA023939	A-431	Nucleoplasm
	HPA023939	U-2 OS	Nucleoplasm
	HPA023939	U-2 OS	Mitochondria	Intensity	Intensity
	HPA023939	U-251 MG	Nucleoplasm

CUSTOM DATA


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Thumbnail	Antibody	Cell line	Assay	Description
	HPA023939	U-2 OS	Image	Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. TRMT12 is mostly expressed at the G1/S checkpoint, and during S and/or G2.

CELL CYCLE
Summary	Expression has cell cycle dependency according to custom assay
Intensity variation	Mitochondria

GENE INFORMATION
Gene name	TRMT12 (HGNC Symbol)
Synonyms	FLJ20772, Trm12, TYW2
Description	TRNA methyltransferase 12 homolog (S. cerevisiae) (HGNC Symbol)
Entrez gene summary	Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]
Chromosome	8
Cytoband	q24.13
Chromosome location (bp)	124450820 - 124462150
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000183665 (version 83.38)
Entrez gene	55039
UniProt	Q53H54 (UniProt - Evidence at protein level)
neXtProt	NX_Q53H54
Antibodypedia	TRMT12 antibodies

PROTEIN BROWSER

TRMT12-001

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
TRMT12-001 ENSP00000329858 ENST00000328599	Q53H54 [Direct mapping] tRNA wybutosine-synthesizing protein 2 homolog Show all	Enzymes ENZYME proteins Transferases SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0006400 [tRNA modification] GO:0008033 [tRNA processing] GO:0010467 [gene expression] GO:0016740 [transferase activity] Show all	448 aa 50.2 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.