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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA023890 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA014704 across 74 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic and membranous positivity in islet cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membrane positivity in glandular cells.
Expression
RNA: detected in 34 tissues Protein: detected in 59 cell types
RNA: detected in 34 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:20
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014704
Antibody HPA023890
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405803)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405803)
Target mass (kDa)
25.8, 15.8
25.8, 22.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA014704
Antibody HPA023890
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
