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ACSM2A

ANTIBODY INFORMATION
 
 

Antibody HPA057699

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA057699  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ACSM2B - ENSG00000066813 [100%]
  

Released in version

 12  

References

  

Proper citation

 Atlas Antibodies Cat#HPA057699, RRID:AB_2683506  

VALIDATION SUMMARY
 
 

Antibody HPA057699

  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057699

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:950  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057699

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.3, 64.2, 13.5, 13.4, 12.1  

Antibody dilution

 1:300  

PROTEIN ARRAY
 
 

Antibody HPA057699

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3600  

ANTIGEN INFORMATION
 
 

Antibody HPA057699

  

Antigen

 Recombinant protein fragment  

Length (aa)

 53  

Antigen sequence

 
LRKVQGLCTLWGTQMSSRTLYINSRQLVSLQWGHQEVPAKFNFASDVLDH
WAD
 

Matching transcripts

 ACSM2A-001 - ENSP00000459451 [100%]
ACSM2A-006 - ENSP00000219054 [100%]
ACSM2A-007 - ENSP00000460349 [100%]
ACSM2A-012 - ENSP00000460948 [100%]
ACSM2A-014 - ENSP00000460031 [100%]
ACSM2A-201 - ENSP00000379411 [100%]
 

Other gene match

 ACSM2B - ENSG00000066813 [100%]
  
ANTIGEN VIEW
ACSM2A-001
ACSM2A-006
ACSM2A-007
ACSM2A-012
ACSM2A-014
ACSM2A-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.