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Immunohistochemical staining of human placenta shows distinct positivity in Hofbauer cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 6 cell types
RNA: detected in 37 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:400
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003281
Antibody CAB011204
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418665)
Target mass (kDa)
19.8, 19.5, 19.1, 17.1
19.1, 16.4, 15.7, 8.2, 8, 7.5, 7.2, 2.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003281
Antibody CAB011204
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
