Fields »
ABOUT
HELP
BLOG
CDCA2

ANTIBODY INFORMATION
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026293   HPA030049  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   6  

References

   1  

Proper citation

 Atlas Antibodies Cat#HPA026293, RRID:AB_1846384   Atlas Antibodies Cat#HPA030049, RRID:AB_10600862  

VALIDATION SUMMARY
 
 

Antibody HPA026293

  

Antibody HPA030049

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA030049.		  
Validated

Antibody staining overlaps with antibody HPA026293.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:50   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear membrane positivity in cells in seminiferous.   Immunohistochemical staining of human testis shows strong nuclear positivity in seminiferus duct cells.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 79 cell types		   RNA: detected in 14 tissues
Protein: detected in 80 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:225  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 112.7, 111   112.7, 111  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA026293

  

Antibody HPA030049

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   94  

Antigen sequence

 
VLSSKRRRISYQRDSDENLTDAEGKVIGLQIFNIDTDRACAVETSVDLSE
ISSKLGSTQSGFLVEESLPLSELTETSNALKVADCVVGKGSS
  
IKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSED
LGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSS
 

Matching transcripts

 CDCA2-001 - ENSP00000328228 [100%]
CDCA2-002 - ENSP00000370040 [100%]
  CDCA2-001 - ENSP00000328228 [100%]
CDCA2-002 - ENSP00000370040 [100%]
 

Other gene match

    
ANTIGEN VIEW
CDCA2-001
CDCA2-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA026293

  

Antibody HPA030049

  

1.

   Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch
Qian J et al
Nat Commun 2015;6:10215
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.