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WT1

ANTIBODY INFORMATION
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA035717   HPA053848   M3561  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 16   12   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA035717, RRID:AB_2674748   Atlas Antibodies Cat#HPA053848, RRID:AB_2682279   Dako Cat#M3561, RRID:AB_2304486  

VALIDATION SUMMARY
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

    Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA053848.		  
Validated

Antibody staining overlaps with antibody HPA035717.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:53   1:33    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human endometrium shows strong nuclear positivity in stromal cells.  

Expression

     RNA: detected in 15 tissues
Protein: detected in 37 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:100  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 56.3, 55.9, 54.3, 34.4, 33.1, 20.1   56.3, 55.9, 54.3   56.3, 55.9, 54.3, 34.4, 33.1, 20.1, 14.2, 3  

Antibody dilution

 1:250   1:110   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:1200    

ANTIGEN INFORMATION
 
 

Antibody HPA035717

  

Antibody HPA053848

  

Antibody CAB000327

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 105   82    

Antigen sequence

 
LGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG
VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQM
HSRKH
  
PHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPP
SQASSGQARMFPNAPYLPSCLESQPAIRNQGY
  

											 
																				

																		

										
											
Matching transcripts

												
																					WT1-001 - ENSP00000331327 [100%] 
WT1-002 - ENSP00000368370 [100%] 
WT1-008 - ENSP00000435624 [100%] 
WT1-009 - ENSP00000413452 [100%] 
WT1-003 - ENSP00000435307 [84%] 
WT1-004 - ENSP00000415516 [84%] 

											 
																						WT1-001 - ENSP00000331327 [100%] 
WT1-004 - ENSP00000415516 [100%] 
WT1-009 - ENSP00000413452 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
WT1-001
WT1-002
WT1-003
WT1-004
WT1-008
WT1-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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