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AP3M1

ANTIBODY INFORMATION
 
 

Antibody HPA045591

  

Antibody HPA048380

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045591   HPA048380  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 AP3M2 - ENSG00000070718 [96%]
   AP3M2 - ENSG00000070718 [96%]
  

Released in version

 14   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045591, RRID:AB_2679377   Atlas Antibodies Cat#HPA048380, RRID:AB_2680379  

VALIDATION SUMMARY
 
 

Antibody HPA045591

  

Antibody HPA048380

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Approved   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045591

  

Antibody HPA048380

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:200  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045591

  

Antibody HPA048380

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47, 46.9, 30.4, 16   47, 46.9, 31.8, 30.4  

Antibody dilution

 1:250   1:220  

PROTEIN ARRAY
 
 

Antibody HPA045591

  

Antibody HPA048380

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1800  

ANTIGEN INFORMATION
 
 

Antibody HPA045591

  

Antibody HPA048380

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 26   25  

Antigen sequence

 
SGDIFLEKHWKSVVSQSVCDYFFEAQ
  
VKYTNNEAYFDVVEEIDAIIDKSGS
 

Matching transcripts

 AP3M1-001 - ENSP00000347408 [100%]
AP3M1-002 - ENSP00000361831 [100%]
  AP3M1-001 - ENSP00000347408 [100%]
AP3M1-002 - ENSP00000361831 [100%]
 

Other gene match

 AP3M2 - ENSG00000070718 [96%]
   AP3M2 - ENSG00000070718 [96%]
  
ANTIGEN VIEW
AP3M1-001
AP3M1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.