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Immunohistochemical staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008888
Antibody CAB004239
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.3, 53.6
57.3, 53.6, 16.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA008888
Antibody CAB004239
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
