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Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Antibody dilution
1:111
1:4
Literature conformity
The subcellular location is supported by literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030943
Antibody HPA030944
Antibody HPA030945
Antibody CAB034184
Standard validation
Approved
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuronal cells and glial cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic and membranous positivity in pancreatic ducts.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells with distinctly stained extracellular material.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 67 cell types
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:500
1:50
1:50
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030943
Antibody HPA030944
Antibody HPA030945
Antibody CAB034184
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
262.9, 257.2, 249
262.9
262.9, 257.2, 249
262.9, 257.2, 249, 62.2, 51.9, 13.6
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA030943
Antibody HPA030944
Antibody HPA030945
Antibody CAB034184
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
