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ANKFY1

ANTIBODY INFORMATION
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024513   HPA024522   HPA065849  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   6   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA024513, RRID:AB_1844830   Atlas Antibodies Cat#HPA024522, RRID:AB_10601453   Atlas Antibodies Cat#HPA065849, RRID:AB_2685567  

VALIDATION SUMMARY
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  
Immunocytochemistry

 

Validated
  

Validated
  
Immunohistochemistry

Approved   Approved    
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA065849.		  
Validated

Antibody staining overlaps with antibody HPA024522.		  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.   Immunofluorescent staining of human cell line A549 shows localization to endosomes.  

Antibody dilution

   1:35   1:95  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human kidney shows strong membranous positivity in tubular cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 28 cell types		   RNA: detected in 37 tissues
Protein: detected in 48 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:200   1:10    

Literature conformity

 Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 132.8, 128.5, 128.4   132.8, 128.5, 128.4   132.8, 128.5, 128.4  

Antibody dilution

 1:250   1:250   1:320  

PROTEIN ARRAY
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:3850  

ANTIGEN INFORMATION
 
 

Antibody HPA024513

  

Antibody HPA024522

  

Antibody HPA065849

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 100   106   82  

Antigen sequence

 
YVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVN
GTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATN
  
SPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNA
NLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDG
SYCYEC
  
ICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGG
CLQTLLHRAIDENNEPTACFLIRSGCDVNSPR
 

Matching transcripts

 ANKFY1-001 - ENSP00000459943 [100%]
ANKFY1-003 - ENSP00000459775 [100%]
ANKFY1-008 - ENSP00000343362 [100%]
  ANKFY1-001 - ENSP00000459943 [100%]
ANKFY1-003 - ENSP00000459775 [100%]
ANKFY1-008 - ENSP00000343362 [100%]
  ANKFY1-001 - ENSP00000459943 [100%]
ANKFY1-003 - ENSP00000459775 [100%]
ANKFY1-008 - ENSP00000343362 [100%]
 

Other gene match

      
ANTIGEN VIEW
ANKFY1-001
ANKFY1-003
ANKFY1-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.