Fields »
ABOUT
HELP
BLOG
SLC36A2

ANTIBODY INFORMATION
 
 

Antibody HPA044002

  

Antibody HPA062229

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044002   HPA062229  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA044002, RRID:AB_10964214   Atlas Antibodies Cat#HPA062229, RRID:AB_2684717  

VALIDATION SUMMARY
 
 

Antibody HPA044002

  

Antibody HPA062229

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044002

  

Antibody HPA062229

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows luminal positivity in renal tubules.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 8 cell types		   RNA: detected in 3 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044002

  

Antibody HPA062229

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 53.2, 43.8   53.2  

Antibody dilution

 1:250   1:270  

PROTEIN ARRAY
 
 

Antibody HPA044002

  

Antibody HPA062229

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3300  

ANTIGEN INFORMATION
 
 

Antibody HPA044002

  

Antibody HPA062229

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 47   23  

Antigen sequence

 
QGAVAIKLDLMSPPESAKKLENKDSTFLDESPSESAGLKKTKGITVF
  
QALDELLKSEDSHPFSNSTTFVR
 

Matching transcripts

 SLC36A2-001 - ENSP00000334223 [100%]
SLC36A2-004 - ENSP00000430535 [100%]
  SLC36A2-001 - ENSP00000334223 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC36A2-001
SLC36A2-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.