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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
Antibody dilution
1:15
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003534
Antibody CAB018380
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human colon shows distinct positivity in endothelial cells.
Immunohistochemical staining of human colon shows moderate positivity in endothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 44 cell types
RNA: detected in 37 tissues Protein: detected in 81 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:1250
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Standard validation
Supported
Figure description
Strong synaptic staining mainly in forebrain, hypothalamus and cerebellum.
WESTERN BLOT
Antibody HPA003534
Antibody CAB018380
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403285)
Target mass (kDa)
12.4, 11.2, 7.9
12.4, 11.2, 7.9, 6.9, 5.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003534
Antibody CAB018380
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
