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ANTIBODY INFORMATION
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015858   HPA029579  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA015858, RRID:AB_1855071   Atlas Antibodies Cat#HPA029579, RRID:AB_10602044  

VALIDATION SUMMARY
 
 

Antibody HPA015858

  

Antibody HPA029579

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane.  

Antibody dilution

   1:400  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate positivity.   Immunohistochemical staining of human cerebral cortex shows moderate positivity.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 65 cell types		   RNA: detected in 23 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:1500  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 560.7, 537.9, 136.4   560.7, 537.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA015858

  

Antibody HPA029579

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 136   121  

Antigen sequence

 
IYQYNYDPSGTASPQTTTEQAILEGQYAALEGSQFWATEDATTTASAVVA
IEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRS
SGAKVRGQYDDMGENMTDDPRSFKKIVDSGVQTDDE
  
PITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLD
RTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEK
DPVKKAKKETGNGIILEVLEA
 

Matching transcripts

 PCLO-001 - ENSP00000334319 [100%]
PCLO-003 - ENSP00000393760 [100%]
PCLO-011 - ENSP00000388393 [100%]
  PCLO-001 - ENSP00000334319 [100%]
PCLO-011 - ENSP00000388393 [100%]
 

Other gene match

    
ANTIGEN VIEW
PCLO-001
PCLO-003
PCLO-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.