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ANTIBODY INFORMATION
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA048895   HPA069524   HPA069570   ab8763  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

        

Released in version

 16   14   14   1  

References

        

Proper citation

 Atlas Antibodies Cat#HPA048895, RRID:AB_2680550   Atlas Antibodies Cat#HPA069524, RRID:AB_2686145   Atlas Antibodies Cat#HPA069570, RRID:AB_2686155    

VALIDATION SUMMARY
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  
Immunocytochemistry

Supported        
Immunohistochemistry

  Supported   Supported  

Validated
  
Western blot

Supported   Supported   Uncertain   Uncertain  
Protein array

Approved   Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Standard validation

 Supported        

       

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.        

Antibody dilution

 1:200        

Literature conformity

 The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

   Supported   Supported   Supported  

       

Figure description

   Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in peripheral ganglia and nerve fibers.   Immunohistochemical staining of human appendix shows positivity in peripheral ganglia and nerve fibers.  

Expression

   RNA: detected in 16 tissues
Protein: detected in 15 cell types		   RNA: detected in 16 tissues
Protein: detected in 48 cell types		   RNA: detected in 16 tissues
Protein: detected in 22 cell types		  

Retrieval

   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

   1:7000   1:300   1:400  

Literature conformity

   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 80.9, 78.9   80.9, 78.9   80.9, 78.9   80.9, 78.9, 45.8, 43, 42.6, 40, 36.8  

Antibody dilution

 1:250   1:390   1:190   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:10000   1:2500   1:2250    

ANTIGEN INFORMATION
 
 

Antibody HPA048895

  

Antibody HPA069524

  

Antibody HPA069570

  

Antibody CAB000151

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 83   83   94    

Antigen sequence

 
PLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTK
EADLPEPSEKQPAAAPRGKPVSRVPQLKARMVS
  
PLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTK
EADLPEPSEKQPAAAPRGKPVSRVPQLKARMVS
  
HAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDS
PPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVS
  

											 
																				

																		

										
											
Matching transcripts

												
																					MAPT-004 - ENSP00000410838 [100%] 
MAPT-008 - ENSP00000458742 [100%] 
MAPT-201 - ENSP00000262410 [100%] 
MAPT-203 - ENSP00000340820 [100%] 

											 
																						MAPT-004 - ENSP00000410838 [100%] 
MAPT-008 - ENSP00000458742 [100%] 
MAPT-201 - ENSP00000262410 [100%] 
MAPT-203 - ENSP00000340820 [100%] 

											 
																						MAPT-004 - ENSP00000410838 [100%] 
MAPT-008 - ENSP00000458742 [100%] 
MAPT-201 - ENSP00000262410 [100%] 
MAPT-203 - ENSP00000340820 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MAPT-004
MAPT-008
MAPT-201
MAPT-203
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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