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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in subset of glandular cells.
Expression
RNA: detected in 7 tissues Protein: detected in 61 cell types
Retrieval
Proteinase K.
Antibody dilution
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Axonal and synaptic positivity observed in selected regions of the brain, most abundant in central amygdala and inner molecular layer of the dentategyrus.
WESTERN BLOT
Antibody HPA045039
Antibody CAB002308
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
12.7
12.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA045039
Antibody CAB002308
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
