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MITF

ANTIBODY INFORMATION
 
 

Antibody HPA003259

  

Antibody CAB002578

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA003259   NCL-L-MITF  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 2   1  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA003259, RRID:AB_1079381    

VALIDATION SUMMARY
 
 

Antibody HPA003259

  

Antibody CAB002578

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003259

  

Antibody CAB002578

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human skin shows strong nuclear positivity in melanocytes.   Immunohistochemical staining of human skin shows strong nuclear positivity in melanocytes.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 11 cell types		   RNA: detected in 36 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH9  

Antibody dilution

 1:50   1:20  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003259

  

Antibody CAB002578

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.8, 58.2, 58, 56.4, 52.5, 46.9, 46.3, 40.4   58.8, 58.2, 58, 56.4, 52.5, 46.9, 46.3, 40.8, 40.4, 18.1, 13.2, 10.4, 8.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003259

  

Antibody CAB002578

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA003259

  

Antibody CAB002578

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 139    

Antigen sequence

 
HLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLL
QHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILM
DDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHT
  

											 
																				

																		

										
											
Matching transcripts

												
																					MITF-001 - ENSP00000295600 [100%] 
MITF-004 - ENSP00000377880 [100%] 
MITF-005 - ENSP00000324246 [100%] 
MITF-007 - ENSP00000391803 [100%] 
MITF-014 - ENSP00000418845 [100%] 
MITF-015 - ENSP00000324443 [100%] 
MITF-016 - ENSP00000435909 [100%] 
MITF-201 - ENSP00000327867 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MITF-001
MITF-004
MITF-005
MITF-007
MITF-014
MITF-015
MITF-016
MITF-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003259

												
										 
																				
											
											
											
Antibody CAB002578

												
										 
																		

																	

										
											
1.

												
																						Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition

	Webster DE et al

	Genome Res 2014;24(5):751-60

			Application: ChIP				
											 
																						
											 
																				

																		

										
											
2.

												
																						MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment

	Riesenberg S et al

	Nat Commun 2015;6:8755

			Application: WB				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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