TMEM72 - Antibodies - The Human Protein Atlas

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TMEM72

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA039894

Antibody HPA062907

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039894

HPA062907

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA039894, RRID:AB_10795261

Atlas Antibodies Cat#HPA062907, RRID:AB_2684900

VALIDATION SUMMARY

Antibody HPA039894

Antibody HPA062907

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA039894

Antibody HPA062907

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA062907 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA039894 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 5 tissues
Protein: detected in 11 cell types

RNA: detected in 5 tissues
Protein: detected in 7 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:150

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039894

Antibody HPA062907

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

29.9, 16.9

Antibody dilution

1:250

1:210

PROTEIN ARRAY

Antibody HPA039894

Antibody HPA062907

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

1:2500

ANTIGEN INFORMATION

Antibody HPA039894

Antibody HPA062907

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

KRKKRKAAPEVLASPEQYTDPSSSAVSTTGSGDTEQTYTFHGALKEGPSS
LFIHMKSILKGTKKPSA

LQPPNTLMELSLEPADSLAKKKQVHFEDNLVRIVPSLAEGLDD

Matching transcripts

TMEM72-001 - ENSP00000374234 [100%]
TMEM72-201 - ENSP00000439911 [100%]

Other gene match

ANTIGEN VIEW

TMEM72-001	TMEM72-201

RELEVANT PUBLICATIONS

Antibody HPA039894

Antibody HPA062907

Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
Fagerberg L et al
Mol Cell Proteomics 2014;13(2):397-406

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.