We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells
Expression
RNA: detected in 20 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017320
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408415)
Target mass (kDa)
78.5
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA017320
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
