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PLSCR1

ANTIBODY INFORMATION
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Thermo Fisher Scientific
  

Product name

 HPA068923   PA5-21649  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA068923, RRID:AB_2686059   Thermo Fisher Scientific Cat#PA5-21649, RRID:AB_11153452  

VALIDATION SUMMARY
 
 

Antibody HPA068923

  

Antibody CAB070421

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

  Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleus, plasma membrane & the Golgi apparatus.    

Antibody dilution

 1:81    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 4 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1300  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Standard validation

   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

   35, 34.2, 27.3, 27.1, 26.8, 19.4, 18.9  

Antibody dilution

   1:500  

PROTEIN ARRAY
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3250    

ANTIGEN INFORMATION
 
 

Antibody HPA068923

  

Antibody CAB070421

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 27    

Antigen sequence

 
MDKQNSQMNASHPETNLPVGYPPQYPP
  

											 
																				

																		

										
											
Matching transcripts

												
																					PLSCR1-001 - ENSP00000345494 [100%] 
PLSCR1-007 - ENSP00000420523 [100%] 
PLSCR1-014 - ENSP00000418103 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PLSCR1-001
PLSCR1-007
PLSCR1-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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