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CLEC2A

ANTIBODY INFORMATION
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048530   HPA072407  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA048530, RRID:AB_2680429   Atlas Antibodies Cat#HPA072407, RRID:AB_2686518  

VALIDATION SUMMARY
 
 

Antibody HPA048530

  

Antibody HPA072407

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.  

Antibody dilution

   1:37  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.    

Expression

 RNA: detected in 2 tissues
Protein: detected in 16 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 20, 18.6   20, 18.6  

Antibody dilution

 1:190   1:120  

PROTEIN ARRAY
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15000   1:1350  

ANTIGEN INFORMATION
 
 

Antibody HPA048530

  

Antibody HPA072407

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 30   26  

Antigen sequence

 
DDTRNWTASKIFCSLQKAELAQIDTQEDME
  
IATWSKHAKPVACSGDWLGVRDKCFY
 

Matching transcripts

 CLEC2A-001 - ENSP00000339732 [100%]
CLEC2A-002 - ENSP00000396163 [100%]
  CLEC2A-001 - ENSP00000339732 [100%]
CLEC2A-002 - ENSP00000396163 [100%]
 

Other gene match

    
ANTIGEN VIEW
CLEC2A-001
CLEC2A-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.