Fields »
ABOUT
HELP
BLOG
NBR1

ANTIBODY INFORMATION
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022944   HPA022999   HPA023999  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA022944, RRID:AB_2671001   Atlas Antibodies Cat#HPA022999, RRID:AB_1859532   Atlas Antibodies Cat#HPA023999, RRID:AB_1859533  

VALIDATION SUMMARY
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.      

Antibody dilution

 1:60      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 31 cell types		   RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:50   1:50  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

      

Target mass (kDa)

 107.4, 103.8, 92.9   107.4, 103.8, 92.9   107.4, 103.8, 92.9  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA022944

  

Antibody HPA022999

  

Antibody HPA023999

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   98   98  

Antigen sequence

 
HEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPA
VQSFPLVPCDTDQPQDKPPDWFTSYLETFR
  
YSTPRLPAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLK
LHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCTSVMPMLS
  
KLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIV
GVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHS
 

Matching transcripts

 NBR1-001 - ENSP00000343479 [100%]
NBR1-002 - ENSP00000437545 [100%]
NBR1-003 - ENSP00000466667 [100%]
NBR1-004 - ENSP00000467816 [100%]
  NBR1-001 - ENSP00000343479 [100%]
NBR1-002 - ENSP00000437545 [100%]
NBR1-003 - ENSP00000466667 [100%]
NBR1-004 - ENSP00000467816 [100%]
  NBR1-001 - ENSP00000343479 [100%]
NBR1-002 - ENSP00000437545 [100%]
NBR1-003 - ENSP00000466667 [100%]
NBR1-004 - ENSP00000467816 [100%]
 

Other gene match

      
ANTIGEN VIEW
NBR1-001
NBR1-002
NBR1-003
NBR1-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.