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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:92
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049421
Antibody HPA066238
Standard validation
Approved
Figure description
Immunohistochemical staining of human cervix, uterine shows moderate nucleolar positivity in squamous epithelial cells.
Expression
RNA: detected in 31 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:1500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049421
Antibody HPA066238
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
200
200
Antibody dilution
1:460
1:1670
PROTEIN ARRAY
Antibody HPA049421
Antibody HPA066238
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
