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ANTIBODY INFORMATION
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA054807   HPA059299   sc-20156  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA054807, RRID:AB_2682607   Atlas Antibodies Cat#HPA059299, RRID:AB_2683970   Santa Cruz Biotechnology Cat#sc-20156, RRID:AB_2137260  

VALIDATION SUMMARY
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA059299.		  
Validated

Antibody staining overlaps with antibody HPA054807.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to mitochondria.   Immunofluorescent staining of human cell line RT4 shows localization to mitochondria.    

Antibody dilution

 1:26   1:23    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 73 cell types		   RNA: detected in 36 tissues
Protein: detected in 50 cell types		   RNA: detected in 36 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:50   1:500  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.7   59.7, 44.8   59.7, 44.8  

Antibody dilution

 1:130   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1050   1:600    

ANTIGEN INFORMATION
 
 

Antibody HPA054807

  

Antibody HPA059299

  

Antibody CAB009437

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 22   41    

Antigen sequence

 
MENQEKASIAGHMFDVVVIGGG
  
VLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVS
  

											 
																				

																		

										
											
Matching transcripts

												
																					MAOA-001 - ENSP00000340684 [100%] 

											 
																						MAOA-001 - ENSP00000340684 [100%] 
MAOA-201 - ENSP00000440846 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MAOA-001
MAOA-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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