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Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic and membrane positivity in squamous epithelial cells.
Expression
RNA: detected in 30 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
Antibody dilution
1:5000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027613
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402803)
Target mass (kDa)
11.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA027613
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
