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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Antibody dilution
1:200
1:66
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA041806
Antibody HPA046289
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human nasopharynx shows strong positivity in respiratory epithelial cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.
Expression
RNA: detected in 27 tissues Protein: detected in 42 cell types
RNA: detected in 27 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:300
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041806
Antibody HPA046289
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423099)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423099)
Target mass (kDa)
44.8, 37.9, 20
44.8, 37.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041806
Antibody HPA046289
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
