TUBB - Antibodies - The Human Protein Atlas

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TUBB

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Abcam plc

Product name

HPA043640

HPA046280

sc-9104

ab6046

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Affinity

Other gene match

TUBB1 - ENSG00000101162 [84%]
TUBB4A - ENSG00000104833 [87%]
TUBB2A - ENSG00000137267 [84%]
TUBB2B - ENSG00000137285 [87%]
TUBB6 - ENSG00000176014 [87%]
TUBB4B - ENSG00000188229 [87%]
RP11-566K11.2 - ENSG00000198211 [84%]
TUBB3 - ENSG00000258947 [84%]
TUBB8 - ENSG00000261456 [100%]

TUBB1 - ENSG00000101162 [85%]
TUBB4A - ENSG00000104833 [93%]
TUBB2A - ENSG00000137267 [96%]
TUBB2B - ENSG00000137285 [96%]
TUBB6 - ENSG00000176014 [93%]
TUBB4B - ENSG00000188229 [96%]
RP11-566K11.2 - ENSG00000198211 [93%]
TUBB3 - ENSG00000258947 [93%]
TUBB8 - ENSG00000261456 [96%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA043640, RRID:AB_2678594

Atlas Antibodies Cat#HPA046280, RRID:AB_2679612

Santa Cruz Biotechnology Cat#sc-9104, RRID:AB_2241191

Abcam Cat#ab6046, RRID:AB_2210370

VALIDATION SUMMARY

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to microtubules.

Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.

Antibody dilution

1:72

1:15

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows neuropil positivity .

Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center.

Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 17 cell types

RNA: detected in 37 tissues
Protein: detected in 65 cell types

RNA: detected in 37 tissues
Protein: detected in 69 cell types

RNA: detected in 37 tissues
Protein: detected in 75 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:2500

1:60

1:7000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Not consistent with RNA expression data.

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

88.4, 50.4, 50.3, 50, 49.9, 49.8, 49.7, 49.6, 47.8, 45.7, 45.5, 42.4, 41.7, 37.4, 20.7, 17.8, 17.4, 15.8

88.4, 50.4, 50.3, 50, 49.9, 49.8, 49.7, 49.6, 47.8, 45.7, 45.5, 42.4, 41.7, 37.4, 20.7, 17.8, 15.8, 12.6

49.7, 47.8, 41.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA043640

Antibody HPA046280

Antibody CAB005417

Antibody CAB012406

Antigen

Recombinant protein fragment

Recombinant protein

Synthetic peptide

Length (aa)

Antigen sequence

SILPSPKVSDTVVEPYNATLSVHQLIENADETFCIDNEALYDICS

GTGSGMGTLLISKIREEYPDRIINTFS

Matching transcripts

TUBB-001 - ENSP00000339001 [87%]
TUBB-002 - ENSP00000365578 [87%]
TUBB-003 - ENSP00000379672 [87%]
TUBB-004 - ENSP00000379668 [87%]

TUBB-001 - ENSP00000339001 [96%]
TUBB-002 - ENSP00000365578 [96%]
TUBB-003 - ENSP00000379672 [96%]
TUBB-004 - ENSP00000379668 [96%]

Other gene match

ANTIGEN VIEW

TUBB-001	TUBB-002	TUBB-003	TUBB-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.