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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody CAB002310

 

Antibody CAB032706

 

Provider

Novocastra
  Santa Cruz Biotechnology
 

Product name

NCL-SERCA2   sc-30110  

Host species

Mouse   Rabbit  

Clonality

mAb   pAb  

Purity

Not known   Protein A/G  

Other gene match

   

Released in version

1   11  

References

   

Proper citation

  Santa Cruz Biotechnology Cat#sc-30110, RRID:AB_2061286  

VALIDATION SUMMARY

 
 

Antibody CAB002310

 

Antibody CAB032706

 
Immunocytochemistry

Supported    
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  

IMMUNOCYTOCHEMISTRY

 
 

Antibody CAB002310

 

Antibody CAB032706

 

Standard validation

Supported    

   

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.    

Antibody dilution

1:25    

Literature conformity

The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY

 
 

Antibody CAB002310

 

Antibody CAB032706

 

Standard validation

  Supported  

   

Figure description

  Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

  RNA: detected in 25 tissues
Protein: detected in 3 cell types
 

Retrieval

  HIER pH6  

Antibody dilution

  1:650  

Literature conformity

  Consistent with extensive gene/protein characterization data.  

RNA consistency

  Consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody CAB002310

 

Antibody CAB032706

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
  Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
 

   

Figure description

   

Target mass (kDa)

110.3, 109.3, 95.2, 13.1, 3, 2.6   110.3, 109.3, 95.2, 13.1, 3, 2.6  

Antibody dilution

1:500   1:500  

ANTIGEN INFORMATION

 
 

Antibody CAB002310

 

Antibody CAB032706

 

Antigen

Not known   Recombinant protein  

Length (aa)

   

Antigen sequence

 
 

Matching transcripts

   

Other gene match

   
 

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