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POM121

ANTIBODY INFORMATION
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Södertörns University College
  

Product name

 HPA043809   HPA049817    

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 POM121C - ENSG00000272391 [100%]
   POMZP3 - ENSG00000146707 [100%]
POM121C - ENSG00000272391 [98%]
    

Released in version

 10   10   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA043809, RRID:AB_2678680   Atlas Antibodies Cat#HPA049817, RRID:AB_2680896    

VALIDATION SUMMARY
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.  

Antibody dilution

   1:93   1:200  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nucelar membranous positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:150   1:1250  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 127.7, 125.1, 100.9, 99.1, 99   127.7, 125.1, 100.9, 99.1, 99, 27.6, 20.6, 12.7   127.7, 100.9, 99  

Antibody dilution

 1:250   1:470   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3750    

ANTIGEN INFORMATION
 
 

Antibody HPA043809

  

Antibody HPA049817

  

Antibody CAB003710

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 55   57    

Antigen sequence

 
HHSSSSTPLAADKESQGEKAADTTPRKKQNSNSQSTPGSSGQRKRKVQLL
PSRRG
  
MVCSPVTLRIAPPDRRFSRSAIPEQIISSTLSSPSSNAPDPCAKETVLSA
LKEKKKK
  

											 
																				

																		

										
											
Matching transcripts

												
																					POM121-001 - ENSP00000405562 [98%] 
POM121-002 - ENSP00000351124 [98%] 
POM121-003 - ENSP00000378687 [98%] 
POM121-201 - ENSP00000393020 [98%] 
POM121-202 - ENSP00000486504 [98%] 

											 
																						POM121-001 - ENSP00000405562 [96%] 
POM121-002 - ENSP00000351124 [96%] 
POM121-003 - ENSP00000378687 [96%] 
POM121-201 - ENSP00000393020 [96%] 
POM121-202 - ENSP00000486504 [96%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					POM121C - ENSG00000272391 [100%]

											 
																						POMZP3 - ENSG00000146707 [100%]
POM121C - ENSG00000272391 [98%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
POM121-001
POM121-002
POM121-003
POM121-201
POM121-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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