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NBPF4

ANTIBODY INFORMATION
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045044   HPA046411   HPA047447   HPA058050  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 NBPF6 - ENSG00000186086 [100%]
   NBPF6 - ENSG00000186086 [100%]
   NBPF6 - ENSG00000186086 [100%]
   NBPF3 - ENSG00000142794 [84%]
NBPF20 - ENSG00000162825 [96%]
NBPF6 - ENSG00000186086 [84%]
NBPF1 - ENSG00000219481 [96%]
NBPF11 - ENSG00000263956 [96%]
NBPF15 - ENSG00000266338 [100%]
NBPF12 - ENSG00000268043 [100%]
NBPF9 - ENSG00000269713 [100%]
NBPF14 - ENSG00000270629 [100%]
AC240274.1 - ENSG00000271254 [96%]
NBPF19 - ENSG00000271383 [92%]
NBPF10 - ENSG00000271425 [100%]
NBPF26 - ENSG00000273136 [88%]
  

Released in version

 13   13   13   13  

References

        

Proper citation

 Atlas Antibodies Cat#HPA045044, RRID:AB_2679196   Atlas Antibodies Cat#HPA046411, RRID:AB_2679657   Atlas Antibodies Cat#HPA047447, RRID:AB_2680051    

VALIDATION SUMMARY
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  

Standard validation

 Uncertain   Uncertain   Uncertain   Uncertain  

       

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows moderate positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows moderate positivity in Leydig cells.   Immunohistochemical staining of human testis shows strong dot like positivity in subset of cells in seminiferus ducts.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 68 cell types		   RNA: detected in 2 tissues
Protein: detected in 16 cell types		   RNA: detected in 2 tissues
Protein: detected in 34 cell types		   RNA: detected in 2 tissues
Protein: detected in 24 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:50   1:300   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

       Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 75.4, 75.2, 72.2, 72.1, 64.8   72.2, 72.1   75.4, 75.2, 72.2, 72.1, 64.8   646.1, 440.4, 435.6, 343.2, 323.9, 167.7, 139.5, 136.2, 130.6, 128, 127.8, 125.7, 113.1, 108.7, 108.6, 108.4, 108.3, 100.1, 100, 99.8, 99.7, 99.5, 99.4, 94.9, 90.5, 88.2, 77.6, 75.4, 75.2, 73, 72.2, 72.1, 71.6, 67.1, 66.9, 66.8, 64.8, 60.7, 60.6, 60.4, 34.2, 34.1, 34, 33.9, 9.2  

Antibody dilution

 1:250   1:250   1:100   1:190  

PROTEIN ARRAY
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000   1:800   1:2300  

ANTIGEN INFORMATION
 
 

Antibody HPA045044

  

Antibody HPA046411

  

Antibody HPA047447

  

Antibody HPA058050

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   32   74   25  

Antigen sequence

 
RLSQELPEVKEQEVPEDSVNEVYLTPSVHHDVSDCHQPYSSTLSSLEDQL
ACSALDVASPTEAACPQGTWSGDLSHH
  
EGKAPVPPRHHDKSNSYRHREVSFLALDEQKV
  
KQSDLEEVKGQETVAPRLSRGPLRVDKHEIPQESLDGCCLTPSILPDLTP
SYHPYWSTLYSFEDKQVSLALVDK
  
MVVSAGPLSSEKAEMNILEINEKLR
 

Matching transcripts

 NBPF4-001 - ENSP00000389237 [100%]
NBPF4-201 - ENSP00000479545 [100%]
  NBPF4-001 - ENSP00000389237 [100%]
  NBPF4-001 - ENSP00000389237 [100%]
NBPF4-201 - ENSP00000479545 [100%]
  NBPF4-001 - ENSP00000389237 [84%]
NBPF4-201 - ENSP00000479545 [84%]
 

Other gene match

 NBPF6 - ENSG00000186086 [100%]
   NBPF6 - ENSG00000186086 [100%]
   NBPF6 - ENSG00000186086 [100%]
   NBPF3 - ENSG00000142794 [84%]
NBPF20 - ENSG00000162825 [96%]
NBPF6 - ENSG00000186086 [84%]
NBPF1 - ENSG00000219481 [96%]
NBPF11 - ENSG00000263956 [96%]
NBPF15 - ENSG00000266338 [100%]
NBPF12 - ENSG00000268043 [100%]
NBPF9 - ENSG00000269713 [100%]
NBPF14 - ENSG00000270629 [100%]
AC240274.1 - ENSG00000271254 [96%]
NBPF19 - ENSG00000271383 [92%]
NBPF10 - ENSG00000271425 [100%]
NBPF26 - ENSG00000273136 [88%]
  
ANTIGEN VIEW
NBPF4-001
NBPF4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.