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Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
Antibody dilution
1:30
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA021545
Antibody HPA021849
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplsmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 34 tissues Protein: detected in 64 cell types
RNA: detected in 34 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:175
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021545
Antibody HPA021849
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411708)
Target mass (kDa)
27.3, 21.9, 21
27.3, 21.9, 19.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA021545
Antibody HPA021849
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
