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ANTIBODY INFORMATION
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA052583   HPA056072   NCL-L-CD10-270  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 14   14   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA052583, RRID:AB_2681872   Atlas Antibodies Cat#HPA056072, RRID:AB_2683027    

VALIDATION SUMMARY
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA056072 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA052583 across 75 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human duodenum shows strong positivity brush border.   Immunohistochemical staining of human small intestine shows membranous positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong positivity in brush border.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 11 cell types		   RNA: detected in 35 tissues
Protein: detected in 12 cell types		   RNA: detected in 35 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:3000   1:400   1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 85.5, 26.2   85.5   85.5, 26.2, 18, 13.4, 10.4, 8.9, 8.8, 1.7  

Antibody dilution

 1:490   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3950   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA052583

  

Antibody HPA056072

  

Antibody CAB000013

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 90   61    

Antigen sequence

 
VLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVA
TENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDK
  
STVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVS
SLSRTYKESRN
  

											 
																				

																		

										
											
Matching transcripts

												
																					MME-001 - ENSP00000418525 [100%] 
MME-004 - ENSP00000419653 [100%] 
MME-005 - ENSP00000417079 [100%] 
MME-006 - ENSP00000353679 [100%] 
MME-007 - ENSP00000420389 [100%] 
MME-008 - ENSP00000420101 [100%] 
MME-201 - ENSP00000478173 [100%] 

											 
																						MME-001 - ENSP00000418525 [100%] 
MME-004 - ENSP00000419653 [100%] 
MME-005 - ENSP00000417079 [100%] 
MME-006 - ENSP00000353679 [100%] 
MME-007 - ENSP00000420389 [100%] 
MME-201 - ENSP00000478173 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MME-001
MME-004
MME-005
MME-006
MME-007
MME-008
MME-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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