CACNA1H - Antibodies - The Human Protein Atlas

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CACNA1H

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA039125

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039125

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA039125, RRID:AB_10795578

VALIDATION SUMMARY

Antibody HPA039125

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA039125

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.

Antibody dilution

1:23

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA039125

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows cytoplasmic positivity in Leydig cells.

Expression

RNA: detected in 32 tissues
Protein: detected in 61 cell types

Retrieval

HIER pH6

Antibody dilution

1:10

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039125

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

259.2, 258.5, 120.9, 119.5, 118.9, 98.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA039125

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12000

ANTIGEN INFORMATION

Antibody HPA039125

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

EEVSHITSSACPWQPTAEPHGPEASPVAGGERDLRRLYSVDAQGFLDKPG
RADEQWRPSAELGSGEPGEAKAWGPEAEPALGARRKKKMSP

Matching transcripts

CACNA1H-001 - ENSP00000334198 [100%]
CACNA1H-002 - ENSP00000455840 [100%]
CACNA1H-003 - ENSP00000457555 [100%]
CACNA1H-004 - ENSP00000454990 [100%]
CACNA1H-005 - ENSP00000454581 [100%]
CACNA1H-006 - ENSP00000455512 [100%]
CACNA1H-201 - ENSP00000351401 [100%]

Other gene match

ANTIGEN VIEW

CACNA1H-001	CACNA1H-002	CACNA1H-003	CACNA1H-004	CACNA1H-005	CACNA1H-006	CACNA1H-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.