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Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Antibody dilution
1:83
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037717
Standard validation
Approved
Figure description
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037717
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
122.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA037717
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
