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Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 9 cell types
RNA: detected in 36 tissues Protein: detected in 11 cell types
RNA: detected in 36 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:6000
1:15000
1:950
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001399
Antibody HPA023884
Antibody CAB004307
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Testis
Target mass (kDa)
40.8, 38.3
40.8, 38.3
40.8, 38.3
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001399
Antibody HPA023884
Antibody CAB004307
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
