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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & mitochondria.
Antibody dilution
1:69
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008069
Antibody CAB078183
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human vagina shows distinct positivity in basal membrane of squamous epithelium.
Expression
RNA: detected in 35 tissues Protein: detected in 49 cell types
RNA: detected in 35 tissues Protein: detected in 22 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:5000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008069
Antibody CAB078183
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
129.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA008069
Antibody CAB078183
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
