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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:4
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA022915
Antibody HPA044345
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA022915
Antibody HPA044345
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407667)
Target mass (kDa)
46
46
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA022915
Antibody HPA044345
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
