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PGAP1

ANTIBODY INFORMATION
 
 

Antibody HPA069704

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA069704  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 13  

References

  

Proper citation

 Atlas Antibodies Cat#HPA069704, RRID:AB_2686177  

VALIDATION SUMMARY
 
 

Antibody HPA069704

  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA069704

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA069704

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 105.4, 18  

Antibody dilution

 1:140  

PROTEIN ARRAY
 
 

Antibody HPA069704

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA069704

  

Antigen

 Recombinant protein fragment  

Length (aa)

 54  

Antigen sequence

 
HLQASLTTFKNSQPVNPKHSRRSEKKSNHHKDSSIHHLRLSANDAEDSLR
MHST
 

Matching transcripts

 PGAP1-001 - ENSP00000346809 [100%]
PGAP1-003 - ENSP00000390555 [100%]
 

Other gene match

  
ANTIGEN VIEW
PGAP1-001
PGAP1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.