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ANTIBODY INFORMATION
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   University of Tampere
  

Product name

 HPA002529   HPA002632   n/a  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   4   5  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA002529, RRID:AB_1857317   Atlas Antibodies Cat#HPA002632, RRID:AB_1857316    

VALIDATION SUMMARY
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  
Immunocytochemistry

 

Validated
   Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by one siRNA.		    

 
   

Figure description

   Whole cells of segmented cells in 10x-images    

Antibody dilution

   1:6    
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

   1:6   1:2000  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA002632 across 69 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA002529 across 69 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:100   1:2000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 102, 26.2   102   102, 26.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002529

  

Antibody HPA002632

  

Antibody CAB019323

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 105   128    

Antigen sequence

 
ARAIKNGKGLHSKKEVPIHRVADISGDTQKAKQFLPFLQRAGRSEAVVEY
VFSGSRLKLYLPKETCLITFLLAGIECPRGARNLPGLVQEGEPFSEEATL
FTKEL
  
TLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLL
NVEHLSAGCPHVTLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEY
LNAQESAKSARLNLWRYGDFRADDADEF
  

											 
																				

																		

										
											
Matching transcripts

												
																					SND1-001 - ENSP00000346762 [100%] 
SND1-016 - ENSP00000419327 [100%] 

											 
																						SND1-001 - ENSP00000346762 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SND1-001
SND1-016
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002529

												
										 
																				
											
											
											
Antibody HPA002632

												
										 
																				
											
											
											
Antibody CAB019323

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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