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ANTIBODY INFORMATION
 
 

Antibody HPA015624

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015624  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

Proper citation

 Atlas Antibodies Cat#HPA015624, RRID:AB_2669245  

VALIDATION SUMMARY
 
 

Antibody HPA015624

  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015624

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.  

Expression

 RNA: detected in 18 tissues
Protein: detected in 18 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015624

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 27.5, 23.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA015624

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA015624

  

Antigen

 Recombinant protein fragment  

Length (aa)

 68  

Antigen sequence

 
PSMVCLAADSKDQAPCKGDSGGPLVCGKGRVLAGVLSFSSRVCTDIFKPP
VATAVAPYVSWIRKVTGR
 

Matching transcripts

 GZMM-001 - ENSP00000264553 [99%]
GZMM-002 - ENSP00000476255 [99%]
 

Other gene match

  
ANTIGEN VIEW
GZMM-001
GZMM-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.