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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ELANE
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ELANE

Gene description

Elastase, neutrophil expressed

Protein class

Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Plasma proteins
Predicted secreted proteins

Predicted localization

Secreted

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Group enriched (pancreatic cancer, stomach cancer)
HPA (cell line):Cell line enriched (THP-1)
HPA (normal tissue):Tissue enriched (bone marrow)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in a subset of bone marrow cells.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining consistent with RNA expression data. Pending external verification.

Reliability score

Supported based on 2 antibodies.
HPA066836 , CAB015409
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Group enriched (pancreatic cancer, stomach cancer)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA066836
CAB015409

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

All cancer tissues were negative except a rare subset of immune cells.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancers were negative.

GENE INFORMATION

Gene name

ELANE (HGNC Symbol)

Synonyms

ELA2, HLE, HNE, NE

Description

Elastase, neutrophil expressed (HGNC Symbol)

Entrez gene summary

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

Chromosome

19

Cytoband

p13.3

Chromosome location (bp)

851014 - 856247

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000197561 (version 83.38)

Entrez gene

1991

UniProt

P08246 (UniProt - Evidence at protein level)

neXtProt

NX_P08246

Antibodypedia

ELANE antibodies

PROTEIN BROWSER

ELANE-001
ELANE-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ELANE-001
ENSP00000466090
ENST00000590230
P08246 [Direct mapping]
Neutrophil elastase
Show all
Enzymes
   ENZYME proteins
   Hydrolases
   Peptidases
   Serine-type peptidases
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Biotech drugs
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0001106 [RNA polymerase II transcription corepressor activity]
GO:0001878 [response to yeast]
GO:0002020 [protease binding]
GO:0002438 [acute inflammatory response to antigenic stimulus]
GO:0004175 [endopeptidase activity]
GO:0004252 [serine-type endopeptidase activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0006508 [proteolysis]
GO:0006874 [cellular calcium ion homeostasis]
GO:0006909 [phagocytosis]
GO:0008201 [heparin binding]
GO:0008233 [peptidase activity]
GO:0009411 [response to UV]
GO:0009986 [cell surface]
GO:0017053 [transcriptional repressor complex]
GO:0019955 [cytokine binding]
GO:0022617 [extracellular matrix disassembly]
GO:0030141 [secretory granule]
GO:0030163 [protein catabolic process]
GO:0030198 [extracellular matrix organization]
GO:0030574 [collagen catabolic process]
GO:0032496 [response to lipopolysaccharide]
GO:0042742 [defense response to bacterium]
GO:0043406 [positive regulation of MAP kinase activity]
GO:0044130 [negative regulation of growth of symbiont in host]
GO:0045079 [negative regulation of chemokine biosynthetic process]
GO:0045415 [negative regulation of interleukin-8 biosynthetic process]
GO:0045416 [positive regulation of interleukin-8 biosynthetic process]
GO:0048661 [positive regulation of smooth muscle cell proliferation]
GO:0050728 [negative regulation of inflammatory response]
GO:0050778 [positive regulation of immune response]
GO:0050832 [defense response to fungus]
GO:0050900 [leukocyte migration]
GO:0050922 [negative regulation of chemotaxis]
GO:0070062 [extracellular exosome]
GO:0070947 [neutrophil mediated killing of fungus]
Show all
267 aa
28.5 kDa
Yes 0
ELANE-201
ENSP00000263621
ENST00000263621
P08246 [Direct mapping]
Neutrophil elastase
Show all
Enzymes
   ENZYME proteins
   Hydrolases
   Peptidases
   Serine-type peptidases
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Biotech drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0001106 [RNA polymerase II transcription corepressor activity]
GO:0002020 [protease binding]
GO:0002438 [acute inflammatory response to antigenic stimulus]
GO:0004175 [endopeptidase activity]
GO:0004252 [serine-type endopeptidase activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0006508 [proteolysis]
GO:0006874 [cellular calcium ion homeostasis]
GO:0006909 [phagocytosis]
GO:0008201 [heparin binding]
GO:0008233 [peptidase activity]
GO:0009411 [response to UV]
GO:0009986 [cell surface]
GO:0017053 [transcriptional repressor complex]
GO:0019955 [cytokine binding]
GO:0022617 [extracellular matrix disassembly]
GO:0030141 [secretory granule]
GO:0030163 [protein catabolic process]
GO:0030198 [extracellular matrix organization]
GO:0030574 [collagen catabolic process]
GO:0042742 [defense response to bacterium]
GO:0043406 [positive regulation of MAP kinase activity]
GO:0045079 [negative regulation of chemokine biosynthetic process]
GO:0045415 [negative regulation of interleukin-8 biosynthetic process]
GO:0045416 [positive regulation of interleukin-8 biosynthetic process]
GO:0048661 [positive regulation of smooth muscle cell proliferation]
GO:0050728 [negative regulation of inflammatory response]
GO:0050922 [negative regulation of chemotaxis]
GO:0070062 [extracellular exosome]
Show all
267 aa
28.5 kDa
Yes 0

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