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AKAP17A

ANTIBODY INFORMATION
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043247   HPA051020  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA043247, RRID:AB_10964776   Atlas Antibodies Cat#HPA051020, RRID:AB_2681314  

VALIDATION SUMMARY
 
 

Antibody HPA043247

  

Antibody HPA051020

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & cytosol.  

Antibody dilution

   1:64  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1000    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 80.7, 51.5   80.7  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:2550  

ANTIGEN INFORMATION
 
 

Antibody HPA043247

  

Antibody HPA051020

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 98   81  

Antigen sequence

 
GEVENKSLVKSFLACLDGKTIKLSGFSDILKVRAAEFKIDFPTRHDWDSF
FRDAKDMNETLPGERPDTIHLEGLPCKWFALKESGSEKPSEDVLVKVF
  
RLLSILLSKKPDDSHTHDELGVAHADLLQPVLDILQTVSSGCVSATTLHP
LGGQPPAGAPKESPAHPEADGAPKSVNGSVA
 

Matching transcripts

 AKAP17A-002 - ENSP00000370660 [100%]
AKAP17A-003 - ENSP00000324827 [100%]
  AKAP17A-003 - ENSP00000324827 [100%]
 

Other gene match

    
ANTIGEN VIEW
AKAP17A-002
AKAP17A-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.