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ANTIBODY INFORMATION
 
 

Antibody CAB016685

  

Antibody CAB022077

  

Provider

 AbFrontier
   Upstate
  

Product name

 LF-PA0173   07-418  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Protein A/G   Protein A/G  

Other gene match

    

Released in version

 4   5  

References

    

Proper citation

 ABFrontier Cat#LF-PA0173, RRID:AB_1618505    

VALIDATION SUMMARY
 
 

Antibody CAB016685

  

Antibody CAB022077

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB016685

  

Antibody CAB022077

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, cytosol & microtubules.  

Antibody dilution

 1:19   1:19  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB016685

  

Antibody CAB022077

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.   Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 78 cell types		   RNA: detected in 36 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:75  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB016685

  

Antibody CAB022077

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 51.5, 37.7, 24.3, 10.1   51.5, 37.7, 24.3, 10.1  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB016685

  

Antibody CAB022077

  

Antigen

 Synthetic peptide   Synthetic peptide  

Length (aa)

    

Antigen sequence

 

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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