Fields »
ABOUT
HELP
BLOG
CD2AP

ANTIBODY INFORMATION
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003267   HPA003326   sc-25272  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   3   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA003267, RRID:AB_1078458   Atlas Antibodies Cat#HPA003326, RRID:AB_1078457   Santa Cruz Biotechnology Cat#sc-25272, RRID:AB_627009  

VALIDATION SUMMARY
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:7   1:95    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA003326 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA003267 across 45 cell lines.		    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA003326 across 67 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA003267 across 67 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:300   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 71.5   71.5   71.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003267

  

Antibody HPA003326

  

Antibody CAB004352

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 127   150    

Antigen sequence

 
WSGTLNNKLGLFPSNFVKELEVTDDGETHEAQDDSETVLAGPTSPIPSLG
NVSETASGSVTQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKPEKPL
ILQSLGPKTQSVEITKTDTEGKIKAKE
  
VDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGG
HSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKA
NTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKEL
  

											 
																				

																		

										
											
Matching transcripts

												
																					CD2AP-001 - ENSP00000352264 [100%] 

											 
																						CD2AP-001 - ENSP00000352264 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD2AP-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.