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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:108
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027451
Antibody HPA027794
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows nucleolar positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 29 tissues Protein: detected in 61 cell types
RNA: detected in 29 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:15
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027451
Antibody HPA027794
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403119)
Target mass (kDa)
60, 17.6
60
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA027451
Antibody HPA027794
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
