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CACNA1E
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA042515

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042515  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

10  

References

 

Proper citation

 

VALIDATION SUMMARY

 
 

Antibody HPA042515

 
Immunocytochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA042515

 

Standard validation

Approved  

 

Figure description

Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.  

Antibody dilution

1:200  

Literature conformity

The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT

 
 

Antibody HPA042515

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.
 

 

Figure description

 

Target mass (kDa)

261.9, 261.7, 259.2, 257.1, 254.6  

Antibody dilution

1:250  

PROTEIN ARRAY

 
 

Antibody HPA042515

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:3000  

ANTIGEN INFORMATION

 
 

Antibody HPA042515

 

Antigen

Recombinant protein fragment  

Length (aa)

76  

Antigen sequence

AHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQR
TPLSLGQREPPWLARPCHGNCDPTQQ
 

Matching transcripts

CACNA1E-001 - ENSP00000356542 [100%]
CACNA1E-002 - ENSP00000481619 [100%]
CACNA1E-003 - ENSP00000356545 [100%]
CACNA1E-006 - ENSP00000353222 [100%]
CACNA1E-202 - ENSP00000351101 [100%]
CACNA1E-203 - ENSP00000356539 [100%]
CACNA1E-204 - ENSP00000483914 [100%]
CACNA1E-201 - ENSP00000350183 [96%]
 

Other gene match

 
ANTIGEN VIEW
CACNA1E-001
CACNA1E-002
CACNA1E-003
CACNA1E-006
CACNA1E-201
CACNA1E-202
CACNA1E-203
CACNA1E-204
 

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