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Immunofluorescent staining of human cell line A549 shows localization to nucleoli fibrillar center & cytosol.
Antibody dilution
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051754
Antibody HPA077474
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center.
Expression
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051754
Antibody HPA077474
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404385)
Target mass (kDa)
21.9, 19.6, 19.3, 17.7, 7.9
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA051754
Antibody HPA077474
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
