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Immunofluorescent staining of human cell line HeLa shows localization to endoplasmic reticulum.
Antibody dilution
1:53
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA055161
Antibody HPA059303
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055161
Antibody HPA059303
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
