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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Antibody dilution
1:93
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001395
Antibody HPA043871
Antibody CAB004607
Antibody CAB015834
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells and basal cells in the seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells and basal cells in the seminiferous ducts.
Immunohistochemical staining of human testis shows distinct positivity in Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:2500
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001395
Antibody HPA043871
Antibody CAB004607
Antibody CAB015834
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
