DDX39B - Antibodies - The Human Protein Atlas

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DDX39B

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA055334

HPA058450

sc-68342

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

DDX39A - ENSG00000123136 [100%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA055334, RRID:AB_2682787

Atlas Antibodies Cat#HPA058450, RRID:AB_2683721

Santa Cruz Biotechnology Cat#sc-68342, RRID:AB_2243237

VALIDATION SUMMARY

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.

Antibody dilution

1:77

1:5

1:31

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.

Immunohistochemical staining of human fallopian tube shows nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 45 cell types

RNA: detected in 37 tissues
Protein: detected in 60 cell types

RNA: detected in 37 tissues
Protein: detected in 79 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:25

1:125

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.1, 49, 48.8, 32.9, 30.5, 28.8, 26.7, 26.5, 25.9, 21.9, 21.3, 20.5, 14.7, 14.4, 14.3, 13.8, 13.5, 11.9, 10.2

49, 48.8, 32.9, 26.7, 26.5, 25.9, 21.9, 20.5, 14.7, 14.3, 13.5

49, 48.8, 32.9, 28.3, 26.7, 26.5, 25.9, 21.9, 21.7, 20.5, 14.7, 14.3, 13.5

Antibody dilution

1:380

1:80

1:500

PROTEIN ARRAY

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3100

1:500

ANTIGEN INFORMATION

Antibody HPA055334

Antibody HPA058450

Antibody CAB034012

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

YVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQ
AKSGMGKTAVFVLATL

MAENDVDNELLDYEDDEVETAAGGD

Matching transcripts

DDX39B-001 - ENSP00000379475 [100%]
DDX39B-002 - ENSP00000416269 [100%]
DDX39B-006 - ENSP00000365347 [100%]
DDX39B-009 - ENSP00000392672 [100%]
DDX39B-010 - ENSP00000405245 [100%]
DDX39B-011 - ENSP00000399841 [100%]
DDX39B-012 - ENSP00000393984 [100%]
DDX39B-013 - ENSP00000405707 [100%]
DDX39B-014 - ENSP00000416350 [100%]
DDX39B-016 - ENSP00000409426 [100%]
DDX39B-017 - ENSP00000399371 [100%]
DDX39B-018 - ENSP00000410313 [100%]

Other gene match

DDX39A - ENSG00000123136 [100%]

ANTIGEN VIEW

DDX39B-001	DDX39B-002	DDX39B-006	DDX39B-009	DDX39B-010	DDX39B-011	DDX39B-012	DDX39B-013	DDX39B-014	DDX39B-016	DDX39B-017	DDX39B-018

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.