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ANTIBODY INFORMATION
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA051505   HPA054758   sc-65239  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA051505, RRID:AB_2681511   Atlas Antibodies Cat#HPA054758, RRID:AB_2682595   Santa Cruz Biotechnology Cat#sc-65239, RRID:AB_2066366  

VALIDATION SUMMARY
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA054758 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA051505 across 46 cell lines.		    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA054758 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA051505 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in granular pattern in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:25   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.6   25.6   25.6  

Antibody dilution

 1:80   1:140   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:550   1:1100    

ANTIGEN INFORMATION
 
 

Antibody HPA051505

  

Antibody HPA054758

  

Antibody CAB016243

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 55   49    

Antigen sequence

 
TDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEI
GPVFT
  
YEYTDYGGLIFNSYILPPLFLEPGDLRLLDVDNRVVLPIEAPIRIIITS
  

											 
																				

																		

										
											
Matching transcripts

												
																					MT-CO2-201 - ENSP00000354876 [98%] 

											 
																						MT-CO2-201 - ENSP00000354876 [94%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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